PacBio HiFi Long-Read Sequencing – MR DNA

πŸ”¬ PacBio HiFi Long-Read Sequencing – MR DNA

Accurate, Full-Length Sequencing for Genomes, Amplicons, and Transcripts

At MR DNA, we offer advanced long-read sequencing using the PacBio Sequel IIe platform, delivering highly accurate HiFi reads ideal for complete genome assemblies, long amplicons, and full-length transcript isoforms. Whether you’re sequencing bacteria, fungi, viruses, or transcriptomes, our long-read workflows deliver deep biological insight with affordable pricing and expert analysis.

We work hard to make long-read sequencing accessible for any project.


🧬 What Is PacBio HiFi Sequencing?

PacBio HiFi reads combine long read lengths (10–25 kb) with Q20+ base accuracy (99.9%), allowing you to:

  • βœ… Assemble complete genomes and circular chromosomes

  • βœ… Sequence full-length amplicons with no need for overlap stitching

  • βœ… Detect structural variants, gene duplications, and mobile elements

  • βœ… Perform isoform-level transcriptomics (Iso-Seq)

  • βœ… Resolve complex or repetitive regions that short reads can’t


πŸ§ͺ MR DNA Long-Read Sequencing Workflow

Step Description
Sample QC DNA or RNA integrity check (Bioanalyzer or TapeStation)
Library Prep SMRTbell library construction for HiFi reads
Sequencing PacBio Sequel IIe (HiFi CCS reads)
Data Delivery FASTQ, FASTA, and optional assembly + annotation

πŸ“Š Available Applications

πŸ”¬ Full-Length Amplicon Sequencing

  • 16S, ITS, or custom targets up to 10–15 kb

  • Great for biodiversity studies and microbial classification

🧬 Whole Genome Sequencing

  • Complete bacterial, fungal, viral, or eukaryotic genome assembly

  • Circular genome closure and plasmid detection

🧠 Transcriptomics (Iso-Seq)

  • Full-length mRNA isoform discovery

  • No fragmentation or reconstruction needed

  • Ideal for eukaryotic gene expression studies

🧬 Structural Variant & Repeat Analysis

  • Detect large insertions, deletions, and tandem repeats

  • Phased haplotypes and methylation analysis available


πŸ’° Affordable Long-Read Sequencing

MR DNA provides flexible, cost-effective PacBio solutions for small and large projects:

  • Transparent pricing with no hidden fees

  • Choice of DNA or RNA applications

  • Optional add-on: genome assembly, annotation, or Iso-Seq analysis

  • Turnaround time: 2–4 weeks, depending on project scope


πŸ“ž Let’s Talk About Your Long-Read Project

Our scientists can help you decide whether long-read or hybrid sequencing is right for your goals β€” and guide you every step of the way.

πŸ“§ Email: info@mrdnalab.com
πŸ“ž Phone: 1-806-789-7984
🌐 Website: www.mrdnalab.com
πŸ“₯ Request a Quote: Contact Us


πŸ”‘ SEO Keywords Embedded:

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